Coding

Part:BBa_K4339008:Design

Designed by: Chloe Singleton   Group: iGEM22_Exeter   (2022-10-09)


MaSp2 6Rep_CT L. hesperus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 193
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence was modified to remove forbidden restriction sites and reduce the number of repetitive motifs to allow for synthesis.


Source

black widow spider Latrodectus hesperus

References